Ua ntej tshaj plaws, peb yuav tsum paub vim li cas ntau dua thiab ntau dua thiab khaws cov khoom siv tau yooj yim, thiab ua kom qhuav dua li ua kom qhuav-qhuav tau dhau los ua AA loj loj.
Cov txheej txheem feem ntau siv rau kev hloov ntawm cov tshuaj reagents kom qhuav hmoov kom tshem tawm noo noo los ua hmoov qhuav. Hauv qhov chaw tsawg-ib puag ncig yog qhov yooj yim hloov, uas yuav cuam tshuam txoj haujlwm ntawm cov protein, yog li khov-kom qhuav yog qhov tsim nyog. Rau cov neeg muaj cov neeg tiv thaiv cov neeg tiv thaiv, cov haujlwm ntawm cov neeg sawv cev sib txawv yuav txawv.
Taw qhia seb cov khoom siv khov kho kom qhuav-kom qhuav tiv thaiv cov neeg sawv cev? Mas muab faib ua tsib pawg:
1. Pho pho, protein ruaj khov tau cuam tshuam ntau heev los ntawm cov protein ntau, nws yuav tsis muaj cov protein ntau, nws yuav muaj qhov ua kom muaj protein ntau, yuav tsum muaj cov tshuaj tiv thaiv zoo, yuav tsum muaj cov tshuaj tiv thaiv isoffrum
2. Sau tus neeg sawv cev, txhawm rau kom ntseeg tau tias cov khoom khov kho ntawm cov khoom los ntawm cov khoom lag luam los ntawm cov khoom lag luam los ntawm cov khoom lag luam los ntawm cov khoom lag luam los ntawm cov khoom lag luam los ntawm cov khoom lag luam los ntawm cov khoom lag luam los ntawm cov khoom lag luam los ntawm cov khoom noj kom zoo thiab sib xws nrog cov protein. Feem ntau siv sau cov neeg sawv cev yog mana cawv, glycine, bovine ntshiab albumin, thiab lwm yam.
3. Cov suab thaj, cov suab thaj yog expients nyob rau hauv cov txheej txheem ntawm cov protein khov-kom qhuav, xws li cov khoom siv hydroxyl, xws li sucrose thiab trehalose.
Plaub, tsis yog-ionic surfactants tuaj yeem txo cov protein prottein, cov feem ntau siv yog kaum ob 80.
5. Ligands, qee zaum qee cov neeg ligands tau ntxiv los txhim kho lub thermodynamic stability ntawm cov protein.